Product Overview Data Sheet Protocol
When deciding between Cynomolgus (Macaca fascicularis) and Rhesus (Macaca mulatta) IgG1 constant regions for antibody engineering, the choice is usually dictated by your specific preclinical drug development goals.
| Feature | Cynomolgus IgG1 | Rhesus IgG1 |
|---|---|---|
| Primary Use Case | Biotherapeutic Tox/PK studies | Virology, Vaccine, and SIV/SHIV research |
| Sequence Identity to Human | High (~90–93%) | High (~90–93%) |
| Inter-species Identity | Highly conserved with Rhesus (98%+) | Highly conserved with Cyno (98%+) |
| Haplotype Diversity | Lower (especially Mauritian cynos) | High (abundant polymorphisms/allotypes) |
| Availability | Standard CRO favorite for biopharma | Common in academic/government facilities |
This vector is a constitutive mammalian expression vector designed to deliver exceptionally high levels of antibody expression. This circular vector features an enhanced, full-length CMV promoter and other expression elements that typically enable higher expression levels. It can be used in suspension-adapted cells, such as Expi293F™ and ExpiCHO™, for transient protein expression. Additionally, it can serve as a Geneticin®-selectable expression plasmid for engineering stable cell lines. The vector carries an ampicillin resistance gene.
Specifications of Antibody Expression Vector| Antibiotic Resistance | Ampicillin (AmpR) |
| Constitutive or Inducible System | Constitutive |
| Delivery Type | Transfection |
| Promoter | CMV |
| Product Type | Mammalian Expression Vector |
| Cloning Method | Restriction Enzyme (5’-AgeI; 3’-XhoI) or Homologous Assembly |
Contents & Storage
- 5.0 µg of pFB-CHIg-rmG1 in Tris-EDTA buffer
- Store at -20°. Vectors are guaranteed stable for 12 months when properly stored.
Materials required for antibody generation and isotype switching
pFB-CLIg-rmk plasmid expressing the Rhesus monkey kappa light chain.
Vector usage
- Entire monoclonal IgG antibodies production: cloning your heavy/light chain variable regions in the cloning sites to preserve the integrity of the heavy/light chain constant region;
- Isotype switching: pFB-CHIg-rmG1 with pFB-CLIg-rmk plasmids are designed to switch a monoclonal antibody from one isotype to another; therefore, maintaining the generated new antibodies with the same antigen affinity (epitopes) but with different effector functions (enhanced or reduced or even disabled ADCC and CDC).
