Product Overview
The Fusion BioLabs DNA Aptamer Library Kit (DAL-01) provides pre-validated ssDNA library oligos and optimized primers to streamline your in vitro selection (SELEX) workflows. Designed to bypass time-consuming library construction and amplification optimization, this kit contains sufficient reagents to perform 2 to 4 independent aptamer development projects (including primary library setup for Round 1 SELEX and secondary library amplification across iterative rounds), depending on your specific experimental design.
Performance Specifications
Binding Affinity
High-specificity target recognition across diverse targets.
Throughput
Typical iterative selection cycle for pool enrichment.
Storage
Shipped on blue ice; stable for 12 months stored properly.
Key Features
- High Efficiency Amplification: Designed and optimized for rapid, specific amplification during SELEX rounds on both Capture SELEX and traditional magnetic bead-based platforms.
- Versatile Strand Separation: Compatible with multiple single-stranded DNA (ssDNA) generation protocols, allowing flexible integration into your lab’s preferred workflow:
- Lambda Exonuclease Digestion: Utilizes 5′-phosphorylated reverse primers for enzymatic digestion.
- Alkaline Denaturation: Utilizes 5′-biotinylated reverse primers with streptavidin-coated magnetic beads.
- Fluorescent Tracking: Includes a 5′-FAM labeled forward primer for downstream quantification and binding monitoring via fluorescence.
Always heat-denature the primary library at 95°C for 5 minutes and immediately snap-cool on ice or room temperature buffer prior to Round 1 selection. This ensures proper secondary structure formation for target binding.
Library Pool Structures
The kit is available with two random region configurations:
| SKU Option | Forward Primer | Random Region | Reverse Primer | Total Length |
|---|---|---|---|---|
| DAL-01 (N62) | 19 bp | N62 (62 nt) | 19 bp | 100 nt |
| DAL-01 (N40) | 18 bp | N40 (40 nt) | 20 bp | 78 nt |
Kit Components
All components are supplied in liquid format:
| Vial | Component | Concentration | Volume |
|---|---|---|---|
| 1 | ssDNA Library Oligos (N62 or N40) | 100 µM | 30 µL |
| 2 | Unmodified Forward Primer | 100 µM | 30 µL |
| 3 | 5′-FAM Labeled Forward Primer | 100 µM | 30 µL |
| 4 | Unmodified Reverse Primer | 100 µM | 30 µL |
| 5 | 5′-Phosphorylated Reverse Primer | 100 µM | 30 µL |
| 6 | 5′-Biotinylated Reverse Primer | 100 µM | 30 µL |
Protocol Flowchart
Flowchart of Magnetic Beads-Based SELEX Technology
Applications
- Primary ssDNA library construction for Round 1 SELEX.
- Secondary library amplification across iterative SELEX selection cycles.
- Aptamer candidate discovery using magnetic bead, microfluidic, or capture-based selection methods.
