Product Overview
Fusion BioLabs offers a validated DNA Aptamer Library Kit for your own DNA aptamer development. This kit has enough ssDNA library oligos and primers for your primary library construction for 1st round SELEX, and secondary library construction for the iterative SELEX of 2-4 independent DNA aptamer development projects depends on your SELEX strategies. With customizable features and robust performance, our DNA Aptamer Library Kit is designed for omitting your library construction optimization and thus facilitating your DNA aptamer development.
DNA Aptamer Library Structure
scheme of DNA aptamer library structure
Key Features
High Efficiency: Library and primer oligos designed for specific and efficient amplification of DNA library for DNA aptamer SELEX using either Capture SELEX platform or traditional magnetic bead-based SELEX platform.
Flexibility and versatility: For single-stranded DNA (ssDNA) generation, the kit could be used either
1) upgrading SELEX technology by using lambda exonuclease digestion or
2) regular strand separation with streptavidin-coated magnetic beads and alkaline denaturation.
Contents & Storage
| Items | Amount | |
| Vial 1 | ssDNA library oligos | 30 µl, 100 µM |
| Vial 2 | 3’-biotinylated capture oligos | 100 µl, 100 µM |
| Vial 3 | forward primer | 30 µl, 100 µM |
| Vial 4 | 5’-FAM labeled forward primer | 30 µl, 100 µM |
| Vial 5 | reverse primer | 30 µl, 100 µM |
| Vial 6 | 5' phosphorylated reverse primer | 30 µl, 100 µM |
| Vial 7 | 5’-biotinylated reverse primer | 30 µl, 100 µM |
- Store at -20°; Vectors are guaranteed stable for 12 months when properly stored.
Kit Usage
- primary library construction for 1st round SELEX.
- Secondary library construction for the iterative SELEX.
