Product Information
TG1 is an amber suppressor strain of E. coli that’s commonly used for screening phage display libraries. Using special production protocols, our TG1 Phage Display Cells are concentrated and stabilized, can undergo multiple freeze-thaw cycles without losing their titer, and are suitable for M13 phage work and phage display protein expression.
- Applications: These cells are perfect for antibody phage display library construction and can be used for M13 phage work, general cloning, and blue/white screening.
Product Specifications
Genotype: [F´ traD36 proA+B+ lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK- mK-)
Titer: 20 OD600/ml, >2.5×1010 cfu/ml
Doubling Time: ~36 min in 2xYT medium at 37ºC at 250 rpm
No Other Bacterial and Phage Contaminations: No colonies are found after plating 20 µl of cells on 2xYT agar plates supplemented with either ampicillin 100 µg/ml, kanamycin 50 µg/ml, or chloramphenicol 34 µg/ml. There is no observable growth in 2xYT liquid medium at 37ºC in the presence of 100 µg/ml ampicillin or 50 µg/ml kanamycin. Bacteria grown overnight (15 h) at 37ºC in liquid 2xYT medium don’t show any sign of M13 bacteriophage lysis and precipitates.
Shipping Conditions: Ships with Dry Ice
Storage Conditions: Store at -80°C.
Reagents Supplied
Competent Cells: 1 x 0.5 ml
Recovery Medium: 9 x 0.5 ml
