Background
Erythropoietin-producing human hepatocellular carcinoma A2 receptor (EphA2) targeting bispecific T cell-engaging (BiTE) antibody is an artificial bispecific T-cell engager (BiTE). It enables a patient’s T cells to recognize malignant B cells. A molecule of EphA2-targeting BiTE combines two binding sites: a CD3 is part of the T cell receptor and an EphA2 site for the target B cells as a tumor antigen against melanoma, lung, brain, and pancreatic cancers. EphA2 targeting BiTE works by linking these two cell types and activating the T cell to exert cytotoxic activity on the target cell.
The phosphorylation of EphA2 determines whether EphA2 acts as a tumor promoter or suppressor. EphA2 can act as a tyrosine kinase and block AKT activation through ephrin-A1 stimulation, thus inhibiting the growth of cancer cells. Conversely, this interaction is blocked in cancer cells when EphA2 is overexpressed and not activated by ephrin-A1;
Product Details
| Reactivity | Human |
| Specificity | Detects human EphA2 and Human CD3ε in direct ELISA. This product is for research use only. |
| Source | CHO cell transient transfection |
| Sequence | Human EphA2 scFv+Human CD3ε scFv+6xHis tag (N-terminus to C-terminus) |
| Purification | Affinity purified from CHO cell culture supernatant |
| Purity | >95% measured by SDS-PAGE. |
| Predicted MW | 54.1 kDa |
| Immunogen | Human EphA2 and Human CD3ε |
| Endotoxin Level | <0.10 EU per 1 µg of the antibody by the LAL method |
| Formulation | 0.2 µm filtered solution in PBS |
| Reconstitution | Reconstitute at 0.5 mg/ml in sterile 1xPBS |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at -20 to -70°C |
| Storage Conditions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 12 months from the date of receipt at -20 to -70°C as supplied. • 1 month at 2 to 8°C under sterile conditions |