Background
Human folate receptor 3 (FOLR3), or folate receptor gamma (FRγ), is involved in up-take of folic acid. Expression of FOLR3 is correlated more strongly with plasma homocysteine (Hcy) than FOLR1 and FOLR2. FOLR3 may decrease plasma Hcy compared with other FOLRs. It was demonstrated that FOLR3 can metabolize both intracellular Hcy and extracellular Hcy. These results indicate that an increase in FOLR3 may effectively ameliorate Hcy in the blood and weaken Hcy-induced toxicity, even in tissues with the low level of FOLR1 and FOLR2 expression.
Product Details
| Source | Human 293 cells (HEK293) |
| Accession # | P41439-1 |
| Sequence | Human FOLR3 (Gln 23 - Ser 245) + 6x His tag (N-terminus to C-terminus) |
| Purification | Affinity and Size-HPLC |
| Purity | >95% as determined by SDS-PAGE |
| Activity | Measured by its binding ability (500 ng) to Folic Acid-BSA with EC50 =0.89 nM |
| Endotoxin Level | <0.10 EU per 1 µg of the antibody by the LAL method |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.5 mg/ml in sterile 1xPBS |
| Predicted Molecular Weight | 26 kDa |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at -20 to -70°C |
| Storage Conditions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 12 months from the date of receipt at -20 to -70°C as supplied. • 1 month at 2 to 8°C under sterile conditions |
References
Kelemen LE, The role of folate receptor alpha in cancer development, progression and treatment: cause, consequence or innocent bystander?. International Journal of Cancer, 2006, 119 (2): 243–250.
Yoshitomi R, Nakayama K, Yamashita S, Kumazoe M, Lin TA, Mei CY, et al., Plasma Homocysteine Concentration is Associated with the Expression Level of Folate Receptor 3. Scientific Reports, 2020, 10 (1): 10283.