Background
Human folate receptor 1 (FOLR1), or folate receptor gamma (FRα), is responsible for binding to folic acid and its derivatives, which becomes crucial during fetal development. FOLR1 is a member of the folate receptor (FOLR) family. Members of this family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells.
FOLR1 can be overexpressed by a number of epithelial-derived tumors including ovarian, breast, renal, lung, colorectal, and brain. Therefore, antibodies to FOLR1 are being developed for use in targeted therapies.
Product Details
| Source | Human 293 cells (HEK293) |
| Accession # | P15328-1 |
| Sequence | Human FOLR1 (Arg 25 - Met 233) + 6x His tag (N-terminus to C-terminus) |
| Purification | Affinity and Size-HPLC |
| Purity | >95% as determined by SDS-PAGE |
| Activity | Measured by its binding ability (500 ng) to Folic Acid-BSA with EC50 =0.9 nM |
| Endotoxin Level | <0.10 EU per 1 µg of the antibody by the LAL method |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.5 mg/ml in sterile 1xPBS |
| Predicted Molecular Weight | 26 kDa |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at -20 to -70°C |
| Storage Conditions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 12 months from the date of receipt at -20 to -70°C as supplied. • 1 month at 2 to 8°C under sterile conditions |
References
Campbell IG, Jones TA, Foulkes WD, Trowsdale J., Folate-binding protein is a marker for ovarian cancer. Cancer Research. 1991, 51 (19): 5329–5338.
Frye RE, Slattery J, Delhey L, Furgerson B, Strickland T, Tippett M, et al., Folinic acid improves verbal communication in children with autism and language impairment: a randomized double-blind placebo-controlled trial”. Molecular Psychiatry, 2018, 23 (2): 247–256.