Background
Leukocyte immunoglobulin-like receptor subfamily B member 1 (LILRB1), also known as CD85j and ILT2, is a transmembrane glycoprotein in the LILR immunoregulatory protein family. LILRB1 is an inhibitory receptor expressed by different leukocyte lineages which specifically interacts with HLA class I (HLA-I) molecules and the UL18 human cytomegalovirus glycoprotein.
The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It controls inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity.
Product Details
| Source | Human 293 cells (HEK293) |
| Accession # | Q8NHL6-1 |
| Sequence | Human LILRB1 (Gly 24 - His 458) + Human IgG1 (Pro100-Lys330) (N-terminus to C-terminus) |
| Purification | Protein A/G purified and size-HPLC |
| Purity | >95% as determined by SDS-PAGE. |
| Activity | Measured by its binding around 75% of 1x105 HSB2 human peripheral blood acute lymphoblastic leukemia cells using 0.5 µg LILRB1. |
| Endotoxin Level | <0.10 EU per 1 µg of the antibody by the LAL method |
| Formulation | 0.2 µm filtered solution in PBS |
| Reconstitution | Reconstitute at 0.5 mg/ml in sterile 1xPBS |
| Predicted Molecular Weight | 73.8 kDa |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at -20 to -70°C |
| Storage Conditions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 12 months from the date of receipt at -20 to -70°C as supplied. • 1 month at 2 to 8°C under sterile conditions |
References
Baía, D., et al., Interaction of the LILRB1 inhibitory receptor with HLA class Ia dimers. Eur. J. Immunol. 2016, 46: 1681–1690.