Background
Tumor necrosis factor receptor 2 (TNFR2), also known as tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) and CD120b, is one of two membrane receptors that binds tumor necrosis factor-alpha (TNFα). This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity.
Targeting of TNRF2 in tumor cells is associated with increased tumor cell death and decreased progression of tumor cell growth. Increased expression of TNFR2 is found in breast cancer, cervical cancer, colon cancer, and renal cancer. A link between the expression of TNRF2 in tumor cells and late-stage cancer has been discovered.
Product Details
| Source | Human 293 cells (HEK293) |
| Accession # | P20333 |
| Sequence | Human TNFR2 (Leu 23 - Asp 257) + 6x His tag (N-terminus to C-terminus) |
| Purification | Protein A/G purified and size-HPLC |
| Purity | >95% as determined by SDS-PAGE |
| Activity | Measured by its ability to TNFa with EC50 22.42 ng/ml |
| Endotoxin Level | <0.10 EU per 1 µg of the antibody by the LAL method |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.5 mg/ml in sterile 1xPBS |
| Predicted Molecular Weight | 26 kDa |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at -20 to -70°C |
| Storage Conditions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 12 months from the date of receipt at -20 to -70°C as supplied. • 1 month at 2 to 8°C under sterile conditions |
References
Matthews, N. & Neale, M.L., Cytotoxicity assays for tumour necrosis factor and lymphotoxin. In: Lymphokines and Interferons: A Practical Approach (eds M. J. Clemens, A. G. Morris and A. J. H. Gearing), 1987, pp. 221-22S. IRL Press, Oxford.