Product Overview
Fusion BioLabs offers a fully validated DNA Aptamer Development Kit (Capture-SELEX module) for your own DNA aptamer development. This kit is a fully validated DNA aptamer development kit, includes all of the reagents up to SELEX procedures using Fusion BioLabs developed capture-SELEX platform.
- ssDNA library oligos and primers for your primary library construction and secondary library construction for the iterative SELEX up to 15 rounds.
- Target-bound and unbound ssDNA aptamer pool partition using streptavidin magnetic beads.
- Counter and/or negative bound ssDNA aptamer pool removal using streptavidin magnetic beads.
- PCR master mix for dsDNA amplification and purification.
- ssDNA aptamer pool preparation using lambda exonuclease digestion.
- ssDNA aptamer pool purification using quick and simple column-based technology.
The DNA Aptamer Development Kit (Capture-SELEX module) is fully optimized for one Capture-SELEX project (enough for 15 rounds of SELEX) to get your DNA aptamer candidates within 4 weeks.
ssDNA Aptamer Pool Preparation for DNA Capture-SELEX
Procedure for selected ssDNA aptamer pool preparation for DNA capture-SELEX
Key Features
Simple and High Efficiency: Library and primer oligos designed for specific and efficient amplification of DNA library for DNA Capture SELEX platform, and selected ssDNA aptamer pool generation.
Flexibility and Versatility: For single-stranded DNA (ssDNA) generation, the kit could be used upgrading SELEX technology via lambda exonuclease digestion, or regular strand separation with streptavidin-coated magnetic beads and alkaline denaturation (not shown above figure).
Contents & Storage
| Items | Description | Amount |
| 1 | DNA Aptamer Library Kit | 1 set |
| 2 | 2xPCR Master Mix | 15 ml |
| 3 | Streptavidin Magnetic Beads | 1 ml |
| 4 | ssDNA pool preparation kit (15 preps) | 1 set |
| 5 | ssDNA pool purification kit (30 preps) | 1 set |
- Store at -20°; reagents are guaranteed stable for 12 months when properly stored.
Kit Usage
- primary ssDNA library construction for 1st round SELEX.
- Secondary ssDNA library construction for the iterative SELEX.
- A fully validated development kit utilizing capture-SELEX platform is available for both small and large molecular targets.