Human scFv phage display library construction Kit

Human scFv phage display library construction Kit

$1,989.00

Human scFv phage display library construction kit

Product Overview

Fusion BioLabs offers a range of library primer sets and phagemid vector combination for antibody phage display and peptide phage display construction. With customizable features and robust performance, our primer sets and phagemid vectors are designed for facilitating phage display library generation as fast as within one week.

 

pAPD-h-scFv is the phagemid vector for construction of a single-chain variable fragment (scFv) library for human antibodies. Here are the key steps involved in constructing such a library:

  • Amplify V genes from cDNA reverse transcript from RNA isolated from peripheral blood lymphocytes (PBL) or lymphoid tissue of non-immunized or immunized donors using PCR primers corresponding to known VH, Vκ, and Vλ gene sequences.
  • Combine VH and VL repertoires to create the scFv construct using a simple two-fragment PCR assembly procedure.
  • Restriction enzyme digestion with SacI/SpeI
  • Ligation of digested and purified scFv fragment into corresponding restriction enzymes(s) digested and purified pAPD-h-scFv

Key Features

High expression efficiency: Engineered for efficient expression and display of antibody fragment scFv on the surface, allowing for easy screening and selection of target molecules.

Flexibility and versatility: One vector for both antibody library construction and downstream antibody fragment expression. No need subcloning into expression vector for downstream application.

 

Specifications of Antibody Phage Display Vector
Antibiotic ResistanceAmpicillin (AmpR)
Constitutive or Inducible SystemInducible for downstream expression
Delivery TypeTransformation
Product TypePhage display vector or Bacterial Expression vector
Cloning Method5’-SacI and 3’-SpeI

 

Contents & Storage

Content of human scFv phage display library construction Kit
Primer Set 1 for amplification of human variable domain of heavy chain (VH) and light chain (Vk and Vλ)
Vial 1100 µl, 10 µMForward Primer mix (13 oligos) for Vk and Vλ amplification
Vial 2100 µl, 10 µMReverse Primer mix (7 oligos) for Vk and Vλ amplification
Vial 3100 µl, 10 µMForward Primer mix (6 oligos) for VH amplification
Vial 4100 µl, 10 µMReverse Primer mix (5 oligos) for VH amplification
Primer Set 2 for cloning/assembly mouse scFv (Format: Vk,λ-long linker-VH)
Vial 5100 µl, 10 µMForward primer for human scFv cloning/assembly
Vial 6100 µl, 10 µMReverse primer for human scFv cloning/assembly
Sequencing Primer set
Vial 7100 µl, 10 µMM13 Reverse primer for scFv forward sequencing
Vial 8100 µl, 10 µMpIII Reverse primer for scFv reverse sequencing
pAPD-h-scFv cloning vector for phage display human scFv library construction
Vial 910.0 µg in Tris-EDTA buffer
  • Store at -20°; Vectors are guaranteed stable for 12 months when properly stored.

 

Vector usage

  • Cloning scFv inserts into pAPD-h-scFv vector for phage display library construction.
  • Cloning scFv candidate into pAPD-h-scFv vector for antibody fragment expression.
Weight 0.5 lbs
Dimensions 1 × 1 × 1 in

DOCUMENTATION

Datasheet

Antibody Phage Library Construction ServicesHigh Quality and Diversity scFv, Fab, VHH Phage Library ConstuctionLearn More
Antibody Phage Library Panning ServicesPhage Library Biopanning and Candidates Validation Learn More
Affibody Development ServicesIdentify Aptamers Using Z-form Affibody Phage LibraryLearn More
Peptide Aptamer Development ServicesIdentify Peptide Aptamers Using Cycliized or Lineraized Peptide Phage library Learn More