The phagemid-based phage display Library has randomized segment of the C8C peptide. C8C peptide is flanked by a pair of cysteine residues, which are oxidized during phage assembly to a disulfide linkage, resulting in the displayed peptides being presented to the target as loops.
- Format: Randomized octapeptide with 6xHis Tag and HA Tag (OmpA leader sequence- cysteine-octapeptide-cysteine-6xHis tag-HA tag-Amber Stop codon)
- Diversity: 7.4×1010
- The diversity and in-frame of the library was checked by NGS.
- Kit contents
The following components are included in the Kit.
Ready-to-panning C8C (Loop-Constrained Octapeptide) Peptide Phage Display Library Kit
| Component | Quantity | Composition |
|---|---|---|
| C8C peptide library (ready-to-panning phage; 7.4×1012pfu/ml) | 1.0 ml | 1×PBS with 25% glycerol |
| M13KO7 Helper Phage (2.0×1012 pfu/ml) | 0.5 ml | 1×PBS with 25% glycerol |
| Chemically Competent TG1 E. coli (2.5x108cfu/ml) | 0.5 ml | 2xYT with 25% Glycerol |
| M13 Reverse Primer (1.6 µM) | 0.2 ml | 1×TE Buffer |
