- Format: Fab with HA Tag and 6x His Tag (OmpA leader sequence-VL-CL : pelB leader sequence-VH-CH1 (human IgG1)-HA HA tag-6xHis tag)
- Diversity: 6.8×1010
- The diversity and in-frame of the library were checked by NGS.
The library was constructed with contributions from various ethnic groups, including Asian, African American, Caucasian, Filipino, and Hispanic, each of which has at least 50 donors aged 25 to 74, with an equal distribution of male and female donors.
- Kit contents
The following components are included in the kit.
Ready-to-panning Human Naïve Fab Library Kit Content
| Component | Quantity | Composition |
|---|---|---|
| Human Naïve Fab library (ready-to-panning phage; 3.2×1012pfu/ml) | 1.0 ml | 1×PBS with 25% glycerol |
| M13KO7 Helper Phage (2.0×1012 pfu/ml) | 0.5 ml | 1×PBS with 25% glycerol |
| Chemically Competent TG1 E. coli (2.5x108cfu/ml) | 0.5 ml | 2xYT with 25% Glycerol |
| M13 Reverse Primer (1.6 µM) | 0.2 ml | 1×TE Buffer |
| pIII Reverse Primer (1.6 µM) | 0.2 ml | 1×TE Buffer |
