- Format: scFv with 6xHis Tag and HA Tag and (OmpA leader sequence-VH-Linker-VL-6xHis tag-HA tag)
- Diversity: 8.9×1010
- The diversity and in-frame of the library was checked by NGS.
The library was constructed by saturation mutagenesis of all 6 CDRs (3 HCDRs and 3 LCDRs) and their CDR combination.
VH Gene Family: IGHV4
VK Gene Family: IGKV2
- Kit contents
The following components are included in the Kit.
Ready-to-panning Human synthetic scFv Library Kit Content
| Component | Quantity | Composition |
|---|---|---|
| Human Synthetic scFv library (ready-to-panning phage; 4.5×1012pfu/ml) | 1.0 ml | 1×PBS with 25% glycerol |
| M13KO7 Helper Phage (2×1012 pfu/ml) | 0.5 ml | 1×PBS with 25% glycerol |
| Chemically Competent TG1 E. coli (2.5x108cfu/ml) | 0.5 ml | 2xYT with 25% Glycerol |
| M13 Reverse Primer (1.6 µM) | 0.2 ml | 1×TE Buffer |
| pIII Reverse Primer (1.6 µM) | 0.2 ml | 1×TE Buffer |
