- Format: scFv with 6xHis Tag and HA Tag and (OmpA leader sequence-VL-Linker-VH-6xHis tag-HA tag)
- Diversity: 2.1×1010
- The diversity and in-frame of the library was checked by NGS.
The library was constructed from spleens of NZW SPF rabbits (New Zealand White rabbits that are Specific Pathogen-Free).
- Kit contents
The following components are included in the Kit.
Ready-to-panning Rabbit Naïve ScFv Library Kit Content
Component | Quantity | Composition |
---|---|---|
Rabbit Naïve scFv library (ready-to-panning phage; 2.1×1012pfu/ml) | 1.0 ml | 1×PBS with 25% glycerol |
M13KO7 Helper Phage (2.0×1012 pfu/ml) | 0.5 ml | 1×PBS with 25% glycerol |
Chemically Competent TG1 E. coli (2.5x108cfu/ml) | 0.5 ml | 2xYT with 25% Glycerol |
M13 Reverse Primer (1.6 µM) | 0.2 ml | 1×TE Buffer |
pIII Reverse Primer (1.6 µM) | 0.2 ml | 1×TE Buffer |