- Format: scFv with C-terminal 6xHis Tag and HA Tag (OmpA leader sequence-VH-Linker-VL-6xHis tag-HA tag)
- Diversity: 9.0×1010
- The diversity and in-frame of the library was checked by NGS.
The Library was constructed from different ethnic groups (Asia, African American, Caucasian, Filipino, Hispanic, and each group has at least 50 donors aged from 25 to 74 with equally distributed male and female.
- Kit contents
The following components are included in the Kit.
Ready-to-panning Human Naïve ScFv Library Kit Content
| Component | Quantity | Composition |
|---|---|---|
| Human Naïve scFv library (ready-to-panning phage; 2.5×1012pfu/ml) | 1.0 ml | 1×PBS with 25% glycerol |
| M13KO7 Helper Phage (2×1012 pfu/ml) | 0.5 ml | 1×PBS with 25% glycerol |
| Chemically Competent TG1 E. coli (2.5x108cfu/ml) | 0.5 ml | 2xYT with 25% Glycerol |
| M13 Reverse Primer (1.6 µM) | 0.2 ml | 1×TE Buffer |
| pIII Reverse Primer (1.6 µM) | 0.2 ml | 1×TE Buffer |
