Peptide Aptamer and Affibody Development Services

Peptide aptamers are small combinatorial proteins that are selected to bind to specific sites on their target molecules. Peptide aptamers consist of short, 5-20 amino acid residues long sequences, typically embedded as a loop within a stable protein scaffold. Fusion Biolabs have 4 ready-to-panning phage display libraries for meet your peptide aptamer development.

Fusion Biolabs have various platforms for peptide aptamer and affibody development.

  1. Phage Display of combinatorial peptide libraries

Phage display is the most widely used approach to identify and isolate high affinity ligands from combinatorial peptide libraries.  Libraries of diversified peptide combinatorial libraries built on artificial scaffolds are introduced directly into the genome of M13 phage by fusing to the gene of the appropriate coat protein pIII.

Fusion Biolabs have four combinatorial peptide libraries for your target screening and biopanning.

Combinatorial Peptide Libraries

Library DescriptionFormatDiversity/Phage titer (pfu/ml) checked by NGSComments
C8CLoop-constrained octapeptide peptide phage display libraryOmpA leader-Csyteine-Octapeptide-Cysteine-6X His tag-HA tag-Amber stop codon7.4x109/7.4X1011Randomized cyclized 8-amino acid peptide with 6xHis Tag and HA Tag
X8Linear 8-amino acid peptide phage display libraryRandomized 8-amino acid peptide with 6xHis Tag and HA Tag (OmpA leader sequence- X8 peptide -6xHis tag-HA tag- Amber stop codon)7.9×109/7.9X1011Randomized 8-amino acid peptide with 6xHis Tag and HA Tag
X16Linear 16-amino acid peptide phage display libraryRandomized 16-amino acid peptide with 6xHis Tag and HA Tag (OmpA leader sequence- X16 peptide-6xHis tag-HA tag-Amber stop codon)8.6×109/8.6X1011Randomized 16-amino acid peptide with 6xHis Tag and HA Tag
X24Linear 24-amino acid peptide phage display libraryRandomized 16-amino acid peptide with 6xHis Tag and HA Tag (OmpA leader sequence- X16 peptide-6xHis tag-HA tag-Amber stop codon)8.2×109/8.2X1111Randomized 16-amino acid peptide with 6xHis Tag and HA Tag
  1. Phage Display of combinatorial Z-format Affibody phage library

Affibody molecules are a class of small robust scaffold proteins derived from the IgG binding domain of Staphylococcus aureus protein A (SPA). Thirteen specific amino acids in the three α-helix regions of the IgG binding domain can be randomly mutated to construct an affibody library. This library can be screened to obtain affibody molecules with high affinity and specificity to any given target molecule.

Z-affibody Library

Library DescriptionFormatDiversity/Phage titer (pfu/ml) checked by NGS
Z-affibodyConstructed by saturation mutagenesis of the Z domain with random amino acid residues at positions 9, 10, 11, 13, 14, 17, 18, 24, 25, 27, 28, 32 and 35.OmpA leader sequence-Z-affibody4.8×109/4.8×1011

To meet your customized inquiry, we also provide other selection techniques to meet your target.

3. Mammalian Cell Surface Display

4. Ribosome Display

5. mRNA Display

Deliverables

1) Sequences of candidate aptamers and affibody (5-16 candidates)
2) Full project report (screening procedures and validation data)

Ordering Information

Peptide aptamer and affibody development services

CatalogDescriptionUnitPrice
92101Peptide Aptamer Development Services1 ProjectContact Us
92102Affibody Development Services1 ProjectContact Us

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Peptide Aptamer and Affibody Development Services