Product Overview
Fusion BioLabs offers a range of library primer sets and phagemid vector combinations for antibody phage display and peptide phage display construction. With customizable features and robust performance, our primer sets and phagemid vectors are designed for facilitating phage display library generation as fast as within one week.
pAPD-VHH is a phagemid vector for the construction of camelid VHH phage display libraries. Here are the key steps involved in constructing such a library:
- Amplify VHH repertoires from cDNA reverse transcript from RNA isolated from peripheral blood lymphocytes (PBL) or lymphoid tissue of non-immunized or immunized donors using PCR Primer Set.
- Restriction enzyme digestion of pAPD-VHH vector and VHH repertoires with NcoI/NotI.
- Ligation of digested and purified VHH repertoires into digested and purified pAPD-VHH vectors to make VHH libraries.
Key Features
High expression efficiency: Engineered for efficient expression and display of antibody fragment scFv on the surface, allowing for easy screening and selection of target molecules.
Flexibility and versatility: One vector for both antibody library construction and downstream antibody fragment expression. No need to subclone into an expression vector for downstream application.
Specifications of Antibody Phage Display Vector
| Antibiotic Resistance | Ampicillin (AmpR) |
| Constitutive or Inducible System | Inducible for downstream expression |
| Delivery Type | Transformation |
| Product Type | Phage display vector or Bacterial Expression vector |
| Cloning Method | NcoI/NotI |
Contents & Storage
Content of VHH phage display library construction Kit| Primer set for amplification of camelid heavy chain antibody (VHH) repertoires | ||
| Vial 1 | 100 µl, 10 µM | Forward (4 oligos) and reverse primer (2 oligos) mix for VHH fragment amplification (normalized concentration) |
| Sequencing primer set | ||
| Vial 2 | 100 µl, 10 µM | M13 Reverse primer for VHH forward sequencing |
| Vial 3 | 100 µl, 10 µM | pIII Reverse primer for VHH reverse sequencing |
| pAPD-VHH cloning vector for phage display VHH library construction | ||
| Vial 4 | 10.0 µg in Tris-EDTA buffer | |
- Store at -20°. Vectors are guaranteed stable for 12 months when properly stored.
Vector usage
- Cloning VHH inserts into the pAPD-VHH vector for VHH phage display library construction.
- Cloning VHH candidate into pAPD-VHH vector for antibody fragment expression.
