Product Overview Data Sheet Protocol
This vector is a constitutive mammalian expression vector designed to deliver exceptionally high levels of antibody expression. This circular vector features an enhanced, full-length CMV promoter and other expression elements that typically enable higher expression levels. It can be used in suspension-adapted cells, such as Expi293F™ and ExpiCHO™, for transient protein expression. Additionally, it can serve as a Geneticin®-selectable expression plasmid for engineering stable cell lines. The vector carries an ampicillin resistance gene.
Specifications of Antibody Expression Vector| Antibiotic Resistance | Ampicillin (AmpR) |
| Constitutive or Inducible System | Constitutive |
| Delivery Type | Transfection |
| Promoter | CMV |
| Product Type | Mammalian Expression Vector |
| Cloning Method | Restriction Enzyme (5’-AgeI; 3’-XhoI) or Homologous Assembly |
Contents & Storage
- 20 µg of pFB-CLIg-hl (human λ2) in Tris-EDTA buffer
- Store at -20°. Vectors are guaranteed stable for 6 months when properly stored.
Materials required for antibody generation and isotype switching
Any plasmid (pFB-CHIg-hG1, pFB-CHIg-hG2, pFB-CHIg-hG3, pFB-CHIg-hG4, pFB-CHIg-hA1, pFB-CHIg-hA2, pFB-CHIg-hD, pFB-CHIg-hE or pFB-CHIg-hM(monoer) and pFB-CHIg-hM (pentamer)) expressing the human heavy chain.
Vector usage
- Entire monoclonal human IgG (IgG1, IgG2, IgG3 and IgG4), IgA, IgD, IgE and IgM antibodies production: cloning your heavy/light chain variable regions in the cloning sites to preserve the integrity of the heavy/light chain constant region.
