Product Overview
Fusion BioLabs offers a range of library primer sets and phagemid vector combination for antibody phage display and peptide phage display construction. With customizable features and robust performance, our primer sets and phagemid vectors are designed for facilitating phage display library generation as fast as within one week.
pAPD-VHH is a phagemid vector for construction of VHH phage display library. Here are the key steps involved in constructing such a library:
- Amplify VHH-hinge repertoires from cDNA reverse transcript from RNA isolated from peripheral blood lymphocytes (PBL) or lymphoid tissue of non-immunized or immunized donors using PCR Primer Set 1.
- Amplify VHH repertoires using Primer Set 2.
- Restriction enzyme digestion pAPD-VHH vector and VHH repertoires obtained from primer set 2 with SfiI.
- Ligation of digested and purified VHH repertoires into digested and purified pAPD-VHH vector to make VHH libraries
Key Features
High expression efficiency: Engineered for efficient expression and display of antibody fragment scFv on the surface, allowing for easy screening and selection of target molecules.
Flexibility and versatility: One vector for both antibody library construction and downstream antibody fragment expression. No need subcloning into expression vector for downstream application.
Specifications of Antibody Phage Display Vector
| Antibiotic Resistance | Ampicillin (AmpR) |
| Constitutive or Inducible System | Inducible for downstream expression |
| Delivery Type | Transformation |
| Product Type | Phage display vector or Bacterial Expression vector |
| Cloning Method | SfiI |
Contents & Storage
Content of VHH phage display library construction Kit| Primer Set 1 | ||
| Vial 1 | 200 µl, 10 µM | Forward and reverse primer mix for VHH-hinge fragment amplification |
| Primer Set 2 | ||
| Vial 2 | 200 µl, 10 µM | Reverse Primer for Vλ fragment amplification |
| pAPD-VHH cloning vector for phage display VHH library construction | ||
| Vial 3 | 10.0 µg in Tris-EDTA buffer | |
- Store at -20°; Vectors are guaranteed stable for 12 months when properly stored.
Vector usage
- Cloning VHH inserts into pAPD-VHH vector for VHH phage display library construction.
- Cloning VHH candidate into pAPD-VHH vector for antibody fragment expression.
