Product Information
SS320 is a non-amber suppressor strain (sometimes called MC1061F’). Use special production protocols, our SS320 Phage Display Cells are concentrated stabilized, could undergo multiple freeze-thaw cycles without losing their titer, are suitable for M13 phage work and phage display protein expression.
- Applications: These cells are perfect for antibody phage display library construction and can be used for M13 phage work, general cloning, blue/white screening, and antibody fragment scFv, Fab and VHH expression.
- IPTG Induction: IPTG induction is required for optimal scFv, Fab, and VHH expression.
Product Specifications
Genotype: [F′proA+B+ lacIqlacZΔM15 Tn10 (tetr)] hsdR mcrB araD139 Δ(araABC-leu)7679 ΔlacX74 galUgalK rpsL thi
Titer: 18 OD600/ml, >2.5×1010 cfu/ml
Doubling Time: ~33 min in 2xYT medium at 37ºC at 250 rpm
No Other Bacterial and Phage Contaminations: No colonies are found after plating 20 µl of cells on 2xYT agar plates supplemented with either ampicillin 100 µg/ml, kanamycin 50 µg/ml, or chloramphenicol 34 µg/ml. There is no observable growth in 2xYT liquid medium at 37ºC in the presence of 100 µg/ml ampicillin or 50 µg/ml kanamycin. Bacteria grew overnight (15 h) at 37ºC in liquid 2xYT medium don’t show any sign of M13 bacteriophage lysis and precipitates.
Shiping Conditions: Ships with Dry Ice
Storage Conditions: Store at -80°C.
Reagents Supplied
Competent Cells: 10 x 0.5 ml
Recovery Media: 5 x 2.0 ml
