pFB-CHIg-hG3: Human IgG3 Mammalian Expression Vector

pFB-CHIg-hG3: Human IgG3 Mammalian Expression Vector

$560.00

Human IgG3 Mammalian Expression Vector

Product Overview                                Data Sheet                   Protocol

This vector is a constitutive mammalian expression vector designed to deliver exceptionally high levels of antibody expression. This circular vector features an enhanced, full-length CMV promoter and other expression elements that typically enable higher expression levels. It can be used in suspension-adapted cells, such as Expi293F™ and ExpiCHO™, for transient protein expression. Additionally, it can serve as a Geneticin®-selectable expression plasmid for engineering stable cell lines. The vector carries an ampicillin resistance gene.

Specifications of Antibody Expression Vector
Antibiotic ResistanceAmpicillin (AmpR)
Constitutive or Inducible SystemConstitutive
Delivery TypeTransfection
PromoterCMV
Product TypeMammalian Expression Vector
Cloning MethodRestriction Enzyme (5’-AgeI; 3’-XhoI) or Homologous Assembly

Contents & Storage

  • 20 µg of pFB-CHIg-hG3 in Tris-EDTA buffer
  • Store at -20°. Vectors are guaranteed stable for 6 months when properly stored.

 

Materials required for antibody generation and isotype switching

pFB-CLIg-hk or pFBCLIg-hl plasmid expressing the human kappa or lambda light chain.

 

Vector usage

  • Entire monoclonal IgG antibodies production: cloning your heavy/light chain variable regions in the cloning sites to preserve the integrity of the heavy/light chain constant region;
  • Isotype switching: from pFB-CHIg-hG3 with pFB-CLIg-hk or pFBCLIg-hl plasmids are designed to switch a monoclonal antibody from one isotype to another, therefore, maintaining the generated new antibodies with the same antigen affinity (epitopes) but with different effector functions (enhanced or reduced or even disabled ADCC and CDC).
Weight 0.2 lbs
Dimensions 1 × 1 × 1 in

Services

Recombinant Antibody ProductionHigh Quality Animal-free Antibody Production via Transient Transfection in CHO or HEK293 PlatformsLearn More
Antibody Fragments ProductionHigh Quality scFv, Fab, VHH Design & Production in Mammalian or Bacterial Expression SystemsLearn More
Biosimilar Antibody ProductionHigh Quality Animal-free Biosimilar Antibody Production via Transient Transfection in CHO Cell PlatformLearn More
Bispecific Antibody ProductionHigh Quality Animal-free Bispecific Antibody Production in Mammalian or Bacterial Expression SystemsLearn More
Antibody Isotype SwitchingSwitching Antibody to any Isotype and Any SpeciesLearn More