Product Information
ER2738 is an amber suppressor strain of E. coli that’s commonly used in phage display library screening. Use special production protocols; our ER2738 Phage Display Cells are concentrated and stabilized, could undergo multiple freeze-thaw cycles without losing their titer, and are suitable for M13 phage work and phage display protein expression.
- Applications: These cells are perfect for antibody phage display library construction and can be used for M13 phage work, general cloning, and blue/white screening.
Product Specifications
Genotype: [F′proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5
Titer: 18 OD600/ml, >2.5×1010 cfu/ml
Doubling Time: ~26 min in 2xYT medium at 37ºC at 250 rpm
No Other Bacterial and Phage Contaminations: No colonies are found after plating 20 µl of cells on 2xYT agar plates supplemented with either ampicillin 100 µg/ml, kanamycin 50 µg/ml, or chloramphenicol 34 µg/ml. There is no observable growth in 2xYT liquid medium at 37ºC in the presence of 100 µg/ml ampicillin or 50 µg/ml kanamycin. Bacteria grown overnight (15 h) at 37ºC in liquid 2xYT medium don’t show any sign of M13 bacteriophage lysis and precipitates.
Shipping Conditions: Ships with Dry Ice
Storage Conditions: Store at -80°C.
Reagents Supplied
Competent Cells: 1 x 0.5 ml
Recovery Media: 9 x 0.5 ml
