Product Information
ER2738 is an amber suppressor strain of E. coli that’s commonly used in phage display library screening. Use special production protocols, our ER2738 Phage Display Cells are concentrated stabilized, could undergo multiple freeze-thaw cycles without losing their titer, are suitable for M13 phage work and phage display protein expression.
- Applications: These cells are perfect for antibody phage display library construction and can be used for M13 phage work, general cloning, blue/white screening.
Product Specifications
Genotype: [F′proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5
Titer: 18 OD600/ml, >2.5×1010 cfu/ml
Doubling Time: ~26 min in 2xYT medium at 37ºC at 250 rpm
No Other Bacterial and Phage Contaminations: No colonies are found after plating 20 µl of cells on 2xYT agar plates supplemented with either ampicillin 100 µg/ml, kanamycin 50 µg/ml, or chloramphenicol 34 µg/ml. There is no observable growth in 2xYT liquid medium at 37ºC in the presence of 100 µg/ml ampicillin or 50 µg/ml kanamycin. Bacteria grew overnight (15 h) at 37ºC in liquid 2xYT medium don’t show any sign of M13 bacteriophage lysis and precipitates.
Shiping Conditions: Ships with Dry Ice
Storage Conditions: Store at -80°C.
Reagents Supplied
Competent Cells: 10 x 0.5 ml
Recovery Media: 5 x 2.0 ml
