Peptide Aptamer and Z-domain-based Scaffold Protein Development Services
Peptide aptamers are small combinatorial proteins that are selected to bind to specific sites on their target molecules. Peptide aptamers consist of short sequences, 5-20 amino acid residues long, typically embedded as a loop within a stable protein scaffold. Fusion Biolabs has 4 ready-to-panning phage display libraries to meet your peptide aptamer development.
Fusion Biolabs has various platforms for peptide aptamer and scaffold protein development.
- Phage Display of combinatorial peptide libraries
Phage display is the most widely used approach to identify and isolate high-affinity ligands from combinatorial peptide libraries. Libraries of diversified peptide combinatorial libraries built on artificial scaffolds are introduced directly into the genome of M13 phage by fusing to the gene of the appropriate coat protein pIII.
Fusion Biolabs has four combinatorial peptide libraries for your target screening and biopanning.
Combinatorial Peptide Libraries
| Library Description | Format | Diversity/Phage titer (pfu/ml) checked by NGS | Comments | |
|---|---|---|---|---|
| C8C | Loop-constrained octapeptide peptide phage display library | OmpA leader-Csyteine-Octapeptide-Cysteine-6X His tag-HA tag-Amber stop codon | 7.4x109/7.4X1011 | Randomized cyclized 8-amino acid peptide with 6xHis Tag and HA Tag |
| X8 | Linear 8-amino acid peptide phage display library | Randomized 8-amino acid peptide with 6xHis Tag and HA Tag (OmpA leader sequence- X8 peptide -6xHis tag-HA tag- Amber stop codon) | 7.9×109/7.9X1011 | Randomized 8-amino acid peptide with 6xHis Tag and HA Tag |
| X16 | Linear 16-amino acid peptide phage display library | Randomized 16-amino acid peptide with 6xHis Tag and HA Tag (OmpA leader sequence- X16 peptide-6xHis tag-HA tag-Amber stop codon) | 8.6×109/8.6X1011 | Randomized 16-amino acid peptide with 6xHis Tag and HA Tag |
| X24 | Linear 24-amino acid peptide phage display library | Randomized 16-amino acid peptide with 6xHis Tag and HA Tag (OmpA leader sequence- X16 peptide-6xHis tag-HA tag-Amber stop codon) | 8.2×109/8.2X1111 | Randomized 16-amino acid peptide with 6xHis Tag and HA Tag |
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Phage Display of combinatorial Z-domain-based scaffold protein phage library
Z-domain-based scaffold proteins are a class of small, robust scaffold proteins derived from the IgG binding domain of Staphylococcus aureus protein A (SPA). Thirteen specific amino acids in the three α-helix regions of the IgG binding domain can be randomly mutated to construct a Z-domain-based scaffold protein library. This library can be screened to obtain Z-domain-based scaffold proteins with high affinity and specificity to any given target molecule.
Z-domain engineered protein scaffold Library
| Library Description | Format | Diversity/Phage titer (pfu/ml) checked by NGS | |
|---|---|---|---|
| Z-domain Engineered Protein Scaffold | aturation mutagenesis at 13 solvent-exposed positions (9, 10, 11, 13, 14, 17, 18, 24, 25, 27, 28, 32, and 35) across Helices 1 and 2 | OmpA leader sequence—Z-domain engineered protein scaffold | 4.8×1011/9.2×1012 |
To meet your customized inquiry, we also provide other selection techniques to meet your target.
3. Mammalian Cell Surface Display
5. mRNA Display
Deliverables
1) Sequences of candidate aptamers and scaffold protein (5-16 candidates)
2) Full project report (screening procedures and validation data)
Ordering Information
Peptide aptamer and Z-domain-based scaffold protein development services
| Catalog | Description | Unit | Price |
|---|---|---|---|
| 92101 | Peptide Aptamer Development Services | 1 Project | Contact Us |
| 92102 | Z-domain-based Scaffold Protein Development Services | 1 Project | Contact Us |
For detailed pricing information, please fill out the form below for a customized quotation or proposal!