Background
Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PD-1 is a cell surface receptor that belongs to the immunoglobulin superfamily and is expressed on T cells and pro-B cells. PD-1 binds two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines.
PD-1 and its ligands negatively regulate immune responses.
Product Details
| Source | Human 293 cells (HEK293) |
| Accession # | Q15116 |
| Sequence | Human PD-1 (Leu 25 - Gln 167) + 6x His tag (N-terminus to C-terminus) |
| Purification | Affinity and Size-HPLC |
| Purity | >95% as determined by SDS-PAGE |
| Activity | Measured by its binding ability (100 ng) to PD-L1 with EC50 =921 ng/ml |
| Endotoxin Level | <0.10 EU per 1 µg of the antibody by the LAL method |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.5 mg/ml in sterile 1xPBS |
| Predicted Molecular Weight | 18.8 kDa |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at -20 to -70°C |
| Storage Conditions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 12 months from the date of receipt at -20 to -70°C as supplied. • 1 month at 2 to 8°C under sterile conditions |
References
Nogrady, B. Immunotherapy: Chemical tricks. Nature 2014, 513, S10–S11.