Background
Interleukin-23 receptor (IL-23R, IL-23 Receptor) is a type I cytokine receptor. In complex with the interleukin-12 receptor β1 subunit (IL-12Rβ1), it is activated by the cytokine interleukin 23 (IL-23). There are 24 IL23R splice variants in mitogen-activated lymphocytes. There is also a variant of IL-23R that consists of just the extracellular part and is known as soluble IL-23R. This form can compete with the membrane-bound form to bind IL-23, modulating the Th17 immune response and regulation of inflammation and immune function.
Three variants in the IL23R gene have been shown to protect against Crohn’s disease and ulcerative colitis.
Product Details
| Source | Human 293 cells (HEK293) |
| Accession # | Q5VWK5-1 |
| Sequence | Human IL-23R (Gly 24 - Gly 355) + 6x His tag (N-terminus to C-terminus) |
| Purification | Affinity and Size-HPLC |
| Purity | >95% as determined by SDS-PAGE |
| Activity | Measured by its binding ability (50 ng) to Anti-Human IL-23R monoclonal antibody with EC50 =0.1-2..0 ng/ml |
| Endotoxin Level | <0.10 EU per 1 µg of the antibody by the LAL method |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.5 mg/ml in sterile 1xPBS |
| Predicted Molecular Weight | 40 kDa |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at -20 to -70°C |
| Storage Conditions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 12 months from the date of receipt at -20 to -70°C as supplied. • 1 month at 2 to 8°C under sterile conditions |
References
Aggarwal S, Ghilardi N, Xie MH, de Sauvage FJ, Gurney AL. Interleukin-23 promotes a distinct CD4 T cell activation state characterized by the production of interleukin-17. J Biol Chem. 2003, 278(3):1910-1914.