Background
Interleukin (IL)-1 Receptor Accessory Protein (IL-1 RAcP), also known as IL-1 R3, is a ubiquitously expressed member of the Interleukin-1 receptor family of proteins. IL-1 R3 is the co-receptor in three signaling pathways that involve six cytokines of the IL-1 family (IL-1α, IL-1β, IL-33, IL-36α, IL-36β and IL-36γ). In many diseases, multiple cytokines contribute to disease pathogenesis. For example, in asthma, both IL-33 and IL-1 are of major importance, as are IL-36 and IL-1 in psoriasis.
Product Details
| Source | Human 293 cells (HEK293) |
| Accession # | Q9NPH3-1 |
| Sequence | Human IL1RAP (Ser 21 - Glu 359) + 6xHis tag (N-terminus to C-terminus) |
| Purification | Affinity and size-HPLC |
| Purity | >95% as determined by SDS-PAGE. |
| Activity | Measured by its binding ability to human IL-1 RI protein (0.2 µg) and Human IL‑1 beta with an EC50=128 ng/ml. |
| Endotoxin Level | <0.10 EU per 1 µg of the antibody by the LAL method |
| Formulation | 0.2 µm filtered solution in PBS |
| Reconstitution | Reconstitute at 0.5 mg/ml in sterile 1xPBS |
| Predicted Molecular Weight | 40 kDa |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at -20 to -70°C |
| Storage Conditions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 12 months from the date of receipt at -20 to -70°C as supplied. • 1 month at 2 to 8°C under sterile conditions |
References
Boraschi D, Tagliabue A. The interleukin-1 receptor family. Semin Immunol. 2013, 25(6):394-407.