Background
Human folate receptor 2 (FOLR2), or folate receptor gamma (FRβ), is a member of the folate receptor (FOLR) family. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells.
FOLR2 has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. The FOLR2 protein exist in placenta, spleen, bone marrow, and thymus.
Product Details
| Source | Human 293 cells (HEK293) |
| Accession # | P14207-1 |
| Sequence | Human FOLR2 (Thr 17 - His 228) + 6x His tag (N-terminus to C-terminus) |
| Purification | Affinity and Size-HPLC |
| Purity | >95% as determined by SDS-PAGE |
| Activity | Measured by its binding ability (500 ng) to Folic Acid-BSA with EC50 =0.82 nM |
| Endotoxin Level | <0.10 EU per 1 µg of the antibody by the LAL method |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.5 mg/ml in sterile 1xPBS |
| Predicted Molecular Weight | 26 kDa |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at -20 to -70°C |
| Storage Conditions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 12 months from the date of receipt at -20 to -70°C as supplied. • 1 month at 2 to 8°C under sterile conditions |
References
Nakashima-Matsushita N, Homma T, Yu S, et al., Selective expression of folate receptor beta and its possible role in methotrexate transport in synovial macrophages from patients with rheumatoid arthritis. Arthritis Rheum, 1999, 42 (8): 1609–1616.
Hao H, Qi H, Ratnam M, Modulation of the folate receptor type beta gene by coordinate actions of retinoic acid receptors at activator Sp1/ets and repressor AP-1 sites. Blood, 2003, 101 (11): 4551–4560.