Name: Ready-to-Use Synthetic VHH Phage Display Library Kit
SKU: ADL-11
Availability: InStock

Format: VHH with HA Tag and 6xHis Tag (pelB leader sequence-VHH-HA tag-6xHis tag)
Framework: ADE99145
Mutated: CDR1, CDR2 and CDR3 (Saturation mutagenesis)
Diversity: 8.9×10¹⁰
The diversity and in-frame of the library was checked by NGS.

The library was constructed by saturation mutagenesis of all 3 CDRs and their CDR combination. The main library consists of 5 sub-libraries with the following CDR composition and saturation mutated CDR combination.

Composition/distribution of CDRs of five (5) VHH sub-libraries

Sub-libraries	CDR1 (7 amnion acids)	CDR2 (6 amino acids)	CDR3 (17 or 23 amino acids)
1	Saturation Mutagenesis	Saturation Mutagenesis	M17 Saturation Mutagenesis
2	Saturation Mutagenesis	Saturation Mutagenesis	M23 Saturation Mutagenesis
3	No Mutagenesis	No Mutagenesis	M17 Saturation Mutagenesis
4	No Mutagenesis	No Mutagenesis	M23 Saturation Mutagenesis
5	No Mutagenesis	Saturation Mutagenesis	M23 Saturation Mutagenesis

Kit contents

The following components are included in the Kit.

Ready-to-panning Synthetic VHH Library Kit Content

Component	Quantity	Composition
Synthetic VHH library (ready-to-panning phage; 8.0×10¹²pfu/ml)	1.0 ml	1×PBS with 25% glycerol
M13KO7 Helper Phage (2.0×10¹² pfu/ml)	0.5 ml	1×PBS with 25% glycerol
Chemically Competent TG1 E. coli (2.5x10⁸cfu/ml)	0.5 ml	2xYT with 25% Glycerol
M13 Reverse Primer (1.6 µM)	0.2 ml	1×TE Buffer
pIII Reverse Primer (1.6 µM)	0.2 ml	1×TE Buffer

Weight	1.0 lbs
Dimensions	1 × 1 × 1 in

DOCUMENTATION

Datasheet Panning Protocol

Services

Antibody Phage Library Construction Services	High Quality and Diversity scFv, Fab, VHH Phage Library Constuction	Learn More
Antibody Phage Library Panning Services	Phage Library Biopanning and Candidates Validation	Learn More
Affibody Development Services	Identify Aptamers Using Z-form Affibody Phage Library	Learn More
Peptide Aptamer Development Services	Identify Peptide Aptamers Using Cycliized or Lineraized Peptide Phage library	Learn More

Ready-to-Use Synthetic VHH Phage Display Library Kit

Ready-to-Use Synthetic VHH Phage Display Library Kit

Composition/distribution of CDRs of five (5) VHH sub-libraries

Ready-to-panning Synthetic VHH Library Kit Content

DOCUMENTATION

Services

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