Name: Ready-to-Use Mouse Synthetic Fab Phage Display Library Kit
SKU: ADL-08
Availability: InStock

Format: Fab with HA Tag and 6xHis Tag (OmpA leader sequence-Vκ-Cκ : pelB leader sequence-VH-CH1 (mouse IgG1)- HA tag-6xHis tag)
Diversity: 3.2×10¹⁰
The diversity and in-frame of the library was checked by NGS.

The mouse synthetic Fab Library was constructed by saturation mutation of all 6 CDRs (3 HCDRs and 3 LCDRs) and their CDR combination.

VH Gene Family: IGHV7

VL Gene Family: IGKV12

The following components are included in the Kit.

Ready-to-panning Mouse synthetic Fab Library Kit Content

Component	Quantity	Composition
Mouse synthetic Fab library (ready-to-panning phage; 3.2×10¹²pfu/ml)	1.0 ml	1×PBS with 25% glycerol
M13KO7 Helper Phage (2×10¹² pfu/ml)	0.5 ml	1×PBS with 25% glycerol
Chemically Competent TG1 E. coli (2.5x10⁸cfu/ml)	0.5 ml	2xYT with 25% Glycerol
M13 Reverse Primer (1.6 µM)	0.2 ml	1×TE Buffer
pIII Reverse Primer (1.6 µM)	0.2 ml	1×TE Buffer

Weight	1.0 lbs
Dimensions	1 × 1 × 1 in

DOCUMENTATION

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