Name: Ready-to-Use Human Synthetic scFv Phage Display Library Kit
SKU: ADL-04
Availability: InStock

Format: scFv with 6xHis Tag and HA Tag and (OmpA leader sequence-VH-Linker-VL-6xHis tag-HA tag)
Diversity: 8.9×10¹⁰
The diversity and in-frame of the library was checked by NGS.

The library was constructed by saturation mutagenesis of all 6 CDRs (3 HCDRs and 3 LCDRs) and their CDR combination.

VH Gene Family: IGHV4

VK Gene Family: IGKV2

The following components are included in the Kit.

Ready-to-panning Human synthetic scFv Library Kit Content

Component	Quantity	Composition
Human Synthetic scFv library (ready-to-panning phage; 4.5×10¹²pfu/ml)	1.0 ml	1×PBS with 25% glycerol
M13KO7 Helper Phage (2×10¹² pfu/ml)	0.5 ml	1×PBS with 25% glycerol
Chemically Competent TG1 E. coli (2.5x10⁸cfu/ml)	0.5 ml	2xYT with 25% Glycerol
M13 Reverse Primer (1.6 µM)	0.2 ml	1×TE Buffer
pIII Reverse Primer (1.6 µM)	0.2 ml	1×TE Buffer

Weight	1.0 lbs
Dimensions	1 × 1 × 1 in

DOCUMENTATION

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