Name: Ready-to-Use Human Synthetic Fab Phage Display Library Kit
SKU: ADL-03
Availability: InStock

Format: Fab with HA Tag and 6xHis Tag (OmpA leader sequence-Vκ-Cκ : pelB leader sequence-VH-CH1 (human IgG1)- HA tag-6xHis tag)
Diversity: 8.0×10¹⁰
The diversity and in-frame of the library was checked by NGS.

The human synthetic Fab Library was constructed by saturation mutation of all 6 CDRs (3 HCDRs and 3 LCDRs) and their CDR combination.

VH Gene Family: IGHV1

VL Gene Family: IGKV4

The following components are included in the Kit.

Ready-to-panning Human synthetic Fab Library Kit Content

Component	Quantity	Composition
Human Synthetic Fab library (ready-to-panning phage; 2.5×10¹²pfu/ml)	1.0 ml	1×PBS with 25% glycerol
M13KO7 Helper Phage (2×10¹² pfu/ml)	0.5 ml	1×PBS with 25% glycerol
Chemically Competent TG1 E. coli (2.5x10⁸cfu/ml)	0.5 ml	2xYT with 25% Glycerol
M13 Reverse Primer (1.6 µM)	0.2 ml	1×TE Buffer
pIII Reverse Primer (1.6 µM)	0.2 ml	1×TE Buffer

Weight	1.0 lbs
Dimensions	1 × 1 × 1 in

DOCUMENTATION

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