Ready-to-Use C8C cyclized peptide phage Display Library Kit

Ready-to-Use C8C cyclized peptide phage Display Library Kit

$3,800.00

Ready-to-panning C8C cyclized peptide phage library kit.

The phagemid-based phage display Library has randomized segment of the C8C peptide. C8C peptide is flanked by a pair of cysteine residues, which are oxidized during phage assembly to a disulfide linkage, resulting in the displayed peptides being presented to the target as loops.

  • Format: Randomized octapeptide with 6xHis Tag and HA Tag (OmpA leader sequence- cysteine-octapeptide-cysteine-6xHis tag-HA tag-Amber Stop codon)
  • Diversity: 7.4×1010
  • The diversity and in-frame of the library was checked by NGS.
  •  Kit contents

The following components are included in the Kit.

Ready-to-panning C8C (Loop-Constrained Octapeptide) Peptide Phage Display Library Kit

ComponentQuantityComposition
C8C peptide library (ready-to-panning phage; 7.4×1012pfu/ml)1.0 ml1×PBS with 25% glycerol
M13KO7 Helper Phage (2.0×1012 pfu/ml)0.5 ml1×PBS with 25% glycerol
Chemically Competent TG1 E. coli (2.5x108cfu/ml)0.5 ml2xYT with 25% Glycerol
M13 Reverse Primer (1.6 µM)0.2 ml1×TE Buffer
Weight 1.0 lbs
Dimensions 1 × 1 × 1 in

DOCUMENTATION

Datasheet                                                                                             Panning Protocol

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