pFB-CHIg-hG4e1: Fc-engineered Human IgG4 Mammalian Expression Vector with Reduced Fab-arm Exchange

pFB-CHIg-hG4e1: Fc-engineered Human IgG4 Mammalian Expression Vector with Reduced Fab-arm Exchange

$560.00

Human IgG4 Mammalian Expression Vector with reduced Fab-arm exchange

Product Overview                                Data Sheet                   Protocol

pFB-CHIg-hG4e1 is a cloning vector that expresses the human IgG4 heavy chain constant region with S228P mutation. It is a constitutive mammalian expression vector designed to deliver exceptionally high levels of antibody expression. This circular vector features an enhanced, full-length CMV promoter and other expression elements that typically enable higher expression levels. It can be used in suspension-adapted cells, such as Expi293F™ and ExpiCHO™, for transient protein expression. Additionally, it can serve as a Geneticin®-selectable expression plasmid for engineering stable cell lines. The vector carries an ampicillin resistance gene.

 

Characteristics

Fc engineered human IgG1 expression with S228P mutation:

–  Reduced Fab-arm exchange

Specifications of Antibody Expression Vector
Antibiotic ResistanceAmpicillin (AmpR)
Constitutive or Inducible SystemConstitutive
Delivery TypeTransfection
PromoterCMV
Product TypeMammalian Expression Vector
Cloning MethodRestriction Enzyme (5’-AgeI; 3’-XhoI) or Homologous Assembly

Contents & Storage

  • 20 µg of pFB-CHIg-hG4e1 in Tris-EDTA buffer
  • Store at -20°. Vectors are guaranteed stable for 6 months when properly stored.

 

Materials required for Fc engineered antibody generation

 

Steps for Fc engineered antibody generation

  • Cloning your heavy chain variable region (VH) into pFB-CHIg-hG4e1 vector to make heavy chain expression plasmid.
  • Cloning your light chain variable region (VL) into pFB-CLIg-hk or pFB-CLIg-hl vector to make light chain expression plasmid.
  • Co-transfecting both heavy chain and light chain expression plasmids into your desired mammalian cell (such as CHO, HEK293) for Fc engineered antibody production.

References

  1. Lee et al., 2019. Publisher Correction: An engineered human Fc domain that behaves like a pH-toggle switch for ultra-long circulation persistence. Nat. Commun. 10, 5461.
  2. Silva et al., 2015. The S228P mutation prevents In Vivo and In Vitro IgG4 Fab-arm exchange as demonstrated using a combination of novel quantitative immunoassays and physiological matrix preparation. J. Biol. Chem. 290, 5462–5469.
Weight 0.2 lbs
Dimensions 1 × 1 × 1 in

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