pFB-CHIg-hG1e2: Fc-engineered Human IgG1 Vector with increased ADCC

pFB-CHIg-hG1e2: Fc-engineered Human IgG1 Vector with increased ADCC

$560.00

Human IgG1 Mammalian Expression Vector with increased ADCC

Product Overview                                Data Sheet                    Protocol

pFB-CHIg-hG1e2 is a cloning vector that expresses the human IgG1 heavy chain constant region with S239D/A330L/I332E mutations. It is a constitutive mammalian expression vector designed to deliver exceptionally high levels of antibody expression. This circular vector features an enhanced, full-length CMV promoter and other expression elements that typically enable higher expression levels. It can be used in suspension-adapted cells, such as Expi293F™ and ExpiCHO™, for transient protein expression. Additionally, it can serve as a Geneticin®-selectable expression plasmid for engineering stable cell lines. The vector carries an ampicillin resistance gene.

 

Characteristics

 Fc engineered human IgG1 expression with S239D/A330L/I332E mutations:

– Increased binding to FcγRIIIa
– Increased ADCC

Specifications of Antibody Expression Vector
Antibiotic ResistanceAmpicillin (AmpR)
Constitutive or Inducible SystemConstitutive
Delivery TypeTransfection
PromoterCMV
Product TypeMammalian Expression Vector
Cloning MethodRestriction Enzyme (5’-AgeI; 3’-XhoI) or Homologous Assembly

Contents & Storage

  • 20 µg of pFB-CHIg-hG1e2 in Tris-EDTA buffer
  • Store at -20° Vectors are guaranteed stable for 6 months when properly stored.

 

Materials required for Fc engineered antibody generation

 

Steps for Fc engineered antibody generation

  • Cloning your heavy chain variable region (VH) into pFB-CHIg-hG1e2 vector to make heavy chain expression plasmid.
  • Cloning your light chain variable region (VL) into pFB-CLIg-hk or pFB-CLIg-hl vector to make light chain expression plasmid.
  • Co-transfecting both heavy chain and light chain expression plasmids into your desired mammalian cell (such as CHO, HEK293) for Fc engineered antibody production.

References

  1. Lazar et al., 2006. Engineered antibody Fc variants with enhanced effector function. Proc. Natl. Acad. Sci. USA 103, 4005–4010.
Weight 0.2 lbs
Dimensions 1 × 1 × 1 in

Services

Recombinant Antibody ProductionHigh Quality Animal-free Antibody Production via Transient Transfection in CHO or HEK293 PlatformsLearn More
Antibody Fragments ProductionHigh Quality scFv, Fab, VHH Design & Production in Mammalian or Bacterial Expression SystemsLearn More
Biosimilar Antibody ProductionHigh Quality Animal-free Biosimilar Antibody Production via Transient Transfection in CHO Cell PlatformLearn More
Bispecific Antibody ProductionHigh Quality Animal-free Bispecific Antibody Production in Mammalian or Bacterial Expression SystemsLearn More
Antibody Isotype SwitchingSwitching Antibody to any Isotype and Any SpeciesLearn More