Product Overview
Fusion BioLabs offers a fully validated DNA Aptamer Development Kit (Capture-SELEX module) for your own DNA aptamer development. This kit is a fully validated DNA aptamer development kit and includes all of the reagents up to SELEX procedures using the Fusion BioLabs-developed capture-SELEX platform.
- ssDNA library oligos and primers for your primary library construction and secondary library construction for the iterative SELEX up to 15 rounds.
- Target-bound and unbound ssDNA aptamer pool partition using streptavidin magnetic beads.
- Counter and/or negative bound ssDNA aptamer pool removal using streptavidin magnetic beads.
- PCR master mix for dsDNA amplification and purification.
- ssDNA aptamer pool preparation using lambda exonuclease digestion.
- ssDNA aptamer pool purification using quick and simple column-based technology.
The DNA Aptamer Development Kit (Capture-SELEX module) is fully optimized for one Capture-SELEX project (enough for 15 rounds of SELEX) to get your DNA aptamer candidates within 4 weeks.
ssDNA Aptamer Pool Preparation for DNA Capture-SELEX
Procedure for selected ssDNA aptamer pool preparation for DNA capture-SELEX
Key Features
Simple and High Efficiency: Library and primer oligos designed for specific and efficient amplification of DNA library for DNA Capture SELEX platform and selected ssDNA aptamer pool generation.
Flexibility and Versatility: For single-stranded DNA (ssDNA) generation, the kit could be used to upgrade SELEX technology via lambda exonuclease digestion or regular strand separation with streptavidin-coated magnetic beads and alkaline denaturation (not shown in the above figure).
Contents & Storage
| Items | Amount |
| Component | Quantity |
| Vial 1: ssDNA Library Oligos | 20 µl, 100 µM |
| Vial 2: 5’-Biotinylated Capture Oligos | 60 µl, 100 µM |
| Vial 3: Forward Primer | 20 µl, 100 µM |
| Vial 4: 5’-FAM Labeled Forward Primer | 20 µl, 100 µM |
| Vial 5: Reverse Primer | 20 µl, 100 µM |
| Vial 6: 5'-Phosphorylated Reverse Primer | 20 µl, 100 µM |
| Vial 7: Streptavidin-Coated Magnetic Beads | 3.0 ml |
| Vial 8: Lambda Exonuclease Reaction Buffer (10X) | 100 µl |
| Vial 9: Lambda Exonuclease (5 units/µl) | 20 µl |
| Vial 10: ssDNA Binding Buffer | 15 ml |
| Vial 11: ssDNA Preparation Buffer | 15 ml |
| Vial 12: ssDNA Wash Buffer | 9.0 ml |
| Vial 13: PCR-grade Water | 1.5 ml |
| Vial 14: 2x PCR Master Mix | 3.0 ml |
| ssDNA Cleanup Column I | 30 columns |
| ssDNA Cleanup Column II | 30 columns |
| Collection Tubes | 60 tubes |
| Nuclease-free Tubes | 30 tubes |
- Store at -20°; reagents are guaranteed stable for 12 months when properly stored.
Kit Usage
- primary ssDNA library construction for 1st round SELEX.
- Secondary ssDNA library construction for the iterative SELEX.
- A fully validated development kit utilizing the capture-SELEX platform is available for both small and large molecular targets.