Background
Dengue virus is a member of the virus family Flaviviridae. It is spread by daytime-active Aedes mosquitoes, such as A. aegypti and A. albopictus. Dengue virus shares a genus with the Zika, yellow fever, Japanese encephalitis, and West Nile viruses. Dengue virus is enveloped and icosahedral and has a non-segmented, single-stranded, 10 kilobase, positive-sense RNA genome. The virus encodes three structural proteins (capsid, membrane and envelope glycoprotein). From these proteins, the envelope glycoprotein, or E protein, has an important role been associated with receptor binding, hemagglutination of erythrocytes, production of neutralizing antibodies and immune response.
In clinical setting, early diagnosis of Dengue infection would be more effective to control epidemic and for timely treatment. A preferred option is to use serological methods for detecting Zika viral antigens (e.g., non-structure proteins). There are seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5), but only NS1 is continuously secreted in blood by the infected host cell, mainly in the first days of infection after the onset. NS1 is a glycoprotein with approximately 46–50 kDa, highly conserved among different serotypes.
Fusion BioLabs provides validated RNA aptamers against non-structure protein (NS-1) of Dengue virus serotype 2. They could be used as direct ELASA alone or paired to capture RNA aptamer and detection RNA aptamer for Sandwich ELASA.
Product Specification
Aptamer type: RNA aptamer (Capture Aptamer and Detection Aptamer Pairs for Sandwich ELASA)
Aptamer length: 61 bp for capture aptamer, 41 bp for detection aptamer
Affinity KD: 37.57 nM (APT-063-10C, 30C for capture aptamer) or 41.40 nM (APT-063-10D, 30D for detection aptamer)
Storage: keep at -80°C. RNA aptamers are guaranteed stable for 12 months when properly stored.