Background
Tumor necrosis factor alpha (TNF, TNFα, TNF-alpha), is a chemical messenger that mediates the immune system and induces inflammation. TNF is produced primarily by activated macrophages and induces inflammation by binding to its target receptors on other cells. It is a member of the tumor necrosis factor superfamily, a family of transmembrane proteins that are immunocytokines, chemical messengers of the immune system.
Excessive production of TNF plays a critical role in several inflammatory diseases, such as rheumatoid arthritis, psoriasis, and inflammatory bowel disease. TNF-blocking drugs that inhibit TNF from binding to its receptors are often employed to treat these diseases. TNF is also implicated in the pathology of other diseases including cancer, liver fibrosis, and Alzheimer’s, although TNF inhibition has yet to show definitive benefits. Due to the important and complex role of TNF in the immune system, the inhibition of TNF can lead to side effects such as increased risk of infections and new forms of autoimmunities.
Product Details
| Source | Human 293 cells (HEK293) |
| Accession # | P20333 |
| Sequence | Human TNFR2 (Leu 23 - Asp 257) + 6x His tag (N-terminus to C-terminus) |
| Purification | Protein A/G purified and size-HPLC |
| Purity | >95% as determined by SDS-PAGE |
| Activity | Measured by its ability to TNFa with EC50 22.42 ng/ml |
| Endotoxin Level | <0.10 EU per 1 µg of the antibody by the LAL method |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.5 mg/ml in sterile 1xPBS |
| Predicted Molecular Weight | 26 kDa |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at -20 to -70°C |
| Storage Conditions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 12 months from the date of receipt at -20 to -70°C as supplied. • 1 month at 2 to 8°C under sterile conditions |
References
Matthews, N. & Neale, M.L., Cytotoxicity assays for tumour necrosis factor and lymphotoxin. In: Lymphokines and Interferons: A Practical Approach (eds M. J. Clemens, A. G. Morris and A. J. H. Gearing), 1987, pp. 221-22S. IRL Press, Oxford.