pFB-CHIg-hG1e4: Fc-engineered Human IgG1 Vector with Reduced ADCC & CDC

pFB-CHIg-hG1e4: Fc-engineered Human IgG1 Vector with Reduced ADCC & CDC

$560.00

Human IgG1 Mammalian Expression Vector with reduced ADCC and CDC

Product Overview                                Data Sheet                    Protocol

pFB-CHIg-hG1e4 is a cloning vector that expresses the human IgG1 heavy chain constant region with E233P/L234V/L235A/ΔG236 + A327G/A330S/P331S mutations. It is a constitutive mammalian expression vector designed to deliver exceptionally high levels of antibody expression. This circular vector features an enhanced, full-length CMV promoter and other expression elements that typically enable higher expression levels. It can be used in suspension-adapted cells, such as Expi293F™ and ExpiCHO™, for transient protein expression. Additionally, it can serve as a Geneticin®-selectable expression plasmid for engineering stable cell lines. The vector carries an ampicillin resistance gene.

 

Characteristics

Fc engineered human IgG1 expression with E233P/L234V/L235A/ΔG236 + A327G/A330S/P331S mutations:

– No binding to FcγRIIb and FcγRIIIa
– Reduced ADCC and CDC

Specifications of Antibody Expression Vector
Antibiotic ResistanceAmpicillin (AmpR)
Constitutive or Inducible SystemConstitutive
Delivery TypeTransfection
PromoterCMV
Product TypeMammalian Expression Vector
Cloning MethodRestriction Enzyme (5’-AgeI; 3’-XhoI) or Homologous Assembly

Contents & Storage

  • 20 µg of pFB-CHIg-hG1e4 in Tris-EDTA buffer
  • Store at -20°. Vectors are guaranteed stable for 6 months when properly stored.

 

Materials required for Fc engineered antibody generation

 

Steps for Fc engineered antibody generation

  • Cloning your heavy chain variable region (VH) into pFB-CHIg-hG1e4 vector to make heavy chain expression plasmid.
  • Cloning your light chain variable region (VL) into pFB-CLIg-hk or pFB-CLIg-hl vector to make light chain expression plasmid.
  • Co-transfecting both heavy chain and light chain expression plasmids into your desired mammalian cell (such as CHO, HEK293) for Fc engineered antibody production.

References

  1. Moore GL et al., 2019. A robust heterodimeric Fc platform engineered for efficient development of bispecific antibodies of multiple formats. Methods 154: 38-50.
Weight 0.2 lbs
Dimensions 1 × 1 × 1 in

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