Ready-to-Use Affibody Phage Display Library Kit

Ready-to-Use Affibody Phage Display Library Kit

$18,500.00

Ready-to-panning Affibody Phage Display Library Kit

Affibody molecules are a class of small robust scaffold proteins derived from the IgG binding domain of Staphylococcus aureus protein A (SPA). Thirteen specific amino acids in the three α-helix regions of the IgG binding domain can be randomly mutated to construct an affibody library. This library can be screened to obtain affibody molecules with high affinity and specificity to any given target molecule.

  • Format: OmpA leader sequence-Z-affibody
  • Diversity: 4.8×1011
  • The diversity and in-frame of the library was checked by NGS.

Fusion BioLabs Z-format Affibody combinatorial ready-to-panning phage library was constructed by saturation mutagenesis of the Z domain with random amino acid residues at positions 9, 10, 11, 13, 14, 17, 18, 24, 25, 27, 28, 32 and 35.

Z-affibody Library

Library DescriptionFormatDiversity/Phage titer (pfu/ml) checked by NGS
Z-affibodyConstructed by saturation mutagenesis of the Z domain with random amino acid residues at positions 9, 10, 11, 13, 14, 17, 18, 24, 25, 27, 28, 32 and 35.OmpA leader sequence-Z-affibody4.8×109/4.8×1011
  •  Kit contents

The following components are included in the Kit.

Ready-to-panning Z-affibody Library Kit Content

ComponentQuantityComposition
Affibody library (ready-to-panning phage; 9.2×1012pfu/ml)1.0 ml1×PBS with 25% glycerol
M13KO7 Helper Phage (2.0×1012 pfu/ml)0.5 ml1×PBS with 25% glycerol
Chemically Competent TG1 E. coli (2.5x108cfu/ml)0.5 ml2xYT with 25% Glycerol
M13 Reverse Primer (1.6 µM)0.2 ml1×TE Buffer
Weight 1.0 lbs
Dimensions 1 × 1 × 1 in

DOCUMENTATION

Datasheet                                                      Panning Protocol

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