Antibody Phage Display Library Construction

An antibody phage display library is a laboratory technique used to study protein-protein, protein-peptide, and protein-DNA interactions. It uses bacteriophages (viruses that infect bacteria) to connect proteins with the genetic information that encodes them. In this technique, a gene encoding a protein of interest is inserted into a phage coat protein gene, causing the phage to “display” the protein on its outside while containing the gene for the protein on its inside, resulting in a connection between genotype and phenotype.

An antibody phage display library is a collection of bacteriophages that display a large number of different antibody fragments on their surface. Phage display or bio-panning allows for the selection of specific antibodies from a large pool of candidates. Fusion Biolabs offer a wide range of formats (scFv, Fab, VHH and affibody) to fit a variety of applications, including non-immune libraries, such as naive antibody phage library or synthetic antibody phage libraries, and immune antibody libraries from immunized animals or patients. Our naive and synthetic libraries generally contain 1 billion to 1 trillion independent clones, while our immune libraries contain at least 5 – 100 billion independent clones. For finding the high specific and affinity clones, you could use our antibody phage display panning service further to meet your need.

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Process Overview

Non-Synthetic Antibody Library Construction Procedure

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Antibody Phage Display Library Construction

Some Citations Used Oak Biosciences/Fusion Biolabs Constructed Libraries

1.Kristopher A Lofgren, Sreeja Sreekumar, E Charles Jenkins Jr, Kyle J Ernzen, Paraic A Kenny, Anti-tumor efficacy of an MMAE-conjugated antibody targeting cell surface TACE/ADAM17-cleaved Amphiregulin in breast cancer, Antibody Therapeutics, Volume 4, Issue 4, October 2021, Pages 252–261, https://doi.org/10.1093/abt/tbab026 (The AREG antibodies were obtained from our naïve rabbit scFv library)

2.ShaniZilberman-Itskovich, RamziaAbu-Hamad, Moshe Stark & Shai Efrati (2019) Effect of anti-C5 antibody on recuperation from ischemia/reperfusion-induced acute kidney injury, Renal Failure, 41:1, 967-975, https://doi.org/ 10.1080/0886022X.2019.1677248 (The anti-C5 antibody was from our human naïve Fab library)

3.Lenka Kyrych Sadilkova, Iva Valentova, Simona Hoskova, Pavel Vopalensky, Lukas Bammert, Roger Beerli, Ulrich Moebius,  Radek Spisek. SO-N102, a novel CLDN18.2-targeting antibody-drug conjugate with strong anti-tumor effect in various solid tumors expressing low target levels. Cancer Res (2021) 81 (13_Supplement): 1204. https://doi.org/10.1158/1538-7445.AM2021-1204 (The anti-CLDN18.2 antibody was from our chicken scFv library and then humanization by us)

4.Chen Y, Jia M, Wang S, Xu S and He N (2022) Antagonistic Antibody Targeting TNFR2 Inhibits Regulatory T Cell Function to Promote Anti-Tumor Activity. Front. Immunol. 13:835690. https://doi.org/10.3389/fimmu.2022.835690 (The anti-TNFR2 antibody was from our Rabbit Immunized scFv library and then humanization by us)

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